AAV Crystal Digital PCR® Assay
Assay-specific Crystal Flex Probes and primers for each of the AAV targets and its Positive Control.
For Research Use Only. Not for use in diagnostic procedures.
Compatibility
Channels
Required Reagents
The AAV Crystal Digital PCR® Assay is a 10X multiplexed assay designed to detect and quantify key regulatory elements of adeno-associated virus (AAV) vectors, enabling vector integrity assessment using the Ruby Chip. Its flexible mix-and-match feature allows customization across diverse cell and gene therapy workflows.
AAV vectors are essential for efficient gene delivery, stable transgene expression, and safety in gene therapy applications. Their manufacturing and purification require stringent quality control to ensure safe and consistent dosing in both clinical trials and patient treatments, as mandated by regulatory agencies.
This assay delivers a robust and precise solution for vector copy number (VCN) quantification, contamination detection, and viral genome integrity assessment—critical parameters for ensuring batch-to-batch consistency in AAV vector production. An indispensable tool for biopharma manufacturers and researchers, it streamlines QC workflows while supporting regulatory compliance in gene therapy development.
This assay is available in 200 and 1000 reaction formats.
Modular "mix-and-match" target selection
Ready-to-use assay reagents to detect multiple targets in a single reaction
Crystal Flex Probe detection chemistry for flexible assay configuration
Same set of Crystal Universal Reporters to detect any target in different assays
ITR2
CMV promoter
WPRE
CMV enhancer
Kanamycin Resitance 3'
Ready-to-use assays & kits
Explore our catalogue of assays and kits and take a leap into the world of multiplexing. Crystal Digital PCR® assays and kits allow for the detection of multiple targets of interests within one single Ruby Chip chamber.
Rapid high-plex assay customization
Stilla offers the fastest assay design service in the market for multiplexed digital PCR assays. Stilla exploits its proprietary Crystal Flex Probe PCR chemistry to offer customized assays in record time, at competitive prices.
