Application note
ESR1 mutation detection in FFPE and Plasma DNA of breast cancer patients using Crystal Digital PCR® and Nio™+.
To detect and quantify the most frequent ESR1 mutations in a single test, a highly multiplexed cdPCR assay was developed: the ESR1 12-plex assay. FFPE DNA and Plasma cfDNA samples from breast cancer patients were analysed using this assay on the Nio™+ system and the results were compared to previously obtained cdPCR results from Centre Eugène Marquis.